Zeiss LSM 980

Inverted laser scanning confocal microscope This confocal is on an Axio Observer 7 inverted microscope. With the Airyscan 2 Module this microscope can scan images at high speed, comparable to that of a spinning disc confocal. Airyscan 2 with 8Ymode is twice as fast as Fast Airyscan mode on the LSM 880s. The Airyscan 2 module allows for super-resolution microscopy and offers a superior signal to noise ratio compared to a normal confocal. The confocal has a 32 channel GaAsP array with two flanking PMTs. The 32 channel GaAsP detector allows for high speed spectral imaging. The Zen Blue Software (version 3.2) allows for automated screening, image analysis and the running of complex experiments. Part of this functionality comes from the Guided Acquisition Module. The black cage incubator allows for very light sensitive applications such as Fluorescence Correlation Spectroscopy (FCS). The incubation chamber provides complete environmental control: Regulating CO2, O2, humidity and temperature. This microscope was installed February 2021. Location: Beckman Institute Basement Room B131.

•    This inverted microscope has the LSM 980 Scanhead with 32 channel GaAsP spectral PMT + 2 MA-PMTs. 1 Detector for transmitted light.
•    Definite Focus 2 for automatic optics based focusing.
•    Fast Airyscan 2 Super-resolution module with it’s own 32 channel GaAsP detector array.
•    7 Laser lines: All solid state lasers.
•    Motorized X,Y stage and Z focus with high speed Piezo insert.
•    Incubation chamber regulates CO2, O2, temperature and humidity.

Technical Specifications: Scope: Zeiss Axio Observer 7 with Definite Focus 2.
Laser Lines: 405, 440, 488, 514, 561, 594 & 639 nm
OS/Software: Windows 10, Zeiss Zen Blue
Removable Media:   DVD Burner, USB, Network Access.